Figure 3
Preleukemic Eμ-myc/E6AP+/− mice have abnormally reduced numbers of pro-B/pre-B cells but normal numbers of sIg+ B cells compared with control Eμ-myc mice. Cells extracted from the BM and spleens of 4-week-old WT (nontransgenic E6AP+/+; ie, WT), E6AP+/−, Eμ-myc, and Eμ-myc/E6AP+/− mice were stained with antibodies to B220, IgM, and IgD and analyzed by FACS. (A) Absolute numbers of total B cells (B220+; *P < .001), mature B cells (IgM+IgD+; **P < .001), and pro-B/pre-B cells (sIg-B220+; ***P < .001) in BM are presented (n = 3/genotype). Values represent mean ± SEM. (B) Absolute numbers of total B cells (B220+; *P < .001), mature B cells (IgM+IgD+; **P < .001), and pro-B/pre-B cells (sIg-B220+; ***P < .001) in the spleen are presented (n = 3/genotype). Values represent mean ± SEM; NS indicates not statistically significant.

Preleukemic Eμ-myc/E6AP+/− mice have abnormally reduced numbers of pro-B/pre-B cells but normal numbers of sIg+ B cells compared with control Eμ-myc mice. Cells extracted from the BM and spleens of 4-week-old WT (nontransgenic E6AP+/+; ie, WT), E6AP+/−, Eμ-myc, and Eμ-myc/E6AP+/− mice were stained with antibodies to B220, IgM, and IgD and analyzed by FACS. (A) Absolute numbers of total B cells (B220+; *P < .001), mature B cells (IgM+IgD+; **P < .001), and pro-B/pre-B cells (sIg-B220+; ***P < .001) in BM are presented (n = 3/genotype). Values represent mean ± SEM. (B) Absolute numbers of total B cells (B220+; *P < .001), mature B cells (IgM+IgD+; **P < .001), and pro-B/pre-B cells (sIg-B220+; ***P < .001) in the spleen are presented (n = 3/genotype). Values represent mean ± SEM; NS indicates not statistically significant.

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