Myc-induced lymphomagenesis is significantly delayed in E6AP heterozygous mice. (A) Death of electronically gated B cells from BM of control wild-type (WT; E6AP^+/+) and E6AP^−/− mice, which had been irradiated with 5 Gy γ-irradiation was measured by PI staining and analyzed by FACS (n = 3, P < .001). Values represent mean ± SD. (B) Kaplan-Meier survival curves of Eμ-myc/E6AP^+/− (n = 36, median survival, 153 days) and control (E6AP^+/+) Eμ-myc transgenic mice (n = 30, median survival 103 days). Nontransgenic E6AP^+/− mice were used as an additional control (n = 30). Lymphomas developed significantly later in Eμ-myc/E6AP^+/− animals compared with the control Eμ-myc mice (P < .001; log-rank test). (C) Numbers of total leukocytes in the blood of sick, lymphoma-burdened mice (n = 15/genotype; P < .01). (D) Weights of spleens of sick, lymphoma burdened mice of the indicated genotypes (n = 15/genotype; P < .001). Values represent mean ± SEM. (E) Weights of combined lymph nodes of sick, lymphoma-burdened mice of the indicated genotypes (n = 15/genotype; P < .01).