Analysis of the TCR-oncogene translocation. (A) Fusion transcripts from TCRδ-TLX1 (T-ALL9) were investigated by PCR and RT-PCR with a range of cDNA and DNA quantities. (B) TCRδ-LMO2 (T-ALL145) and TCRδ-TAL1 (T-ALL437) translocations were investigated by PCR and RT-PCR with a range of cDNA and DNA quantities (positions of oligonucleotide primers are indicated by arrows on upper diagrams). The absence of genomic DNA contamination in the cDNA fraction was validated by quantitative RT-PCR using albumin DNA-specific oligonucleotide primers (not shown) and a RT-negative control was performed for T-ALL9. (C) TLX1, LMO2, and TAL1 quantification by quantitative RT-PCR.