MPL cooperates with RUNX1-ETO in leukemia development in mice. (A) Experimental design. Top: diagram of retroviral constructs used in the transplantation experiment. Bottom: the transduction-transplantation assay. BM cells (circles) harvested from wild-type mice are cotransduced with MIG-R1E, MIG-R1E9a, or MIG, and MID or MID-Mpl retroviruses were GFP-sorted and transplanted into irradiated recipient mice. (B) Kaplan-Meier plot showing the survival of mice transplanted with BM cells transduced with MIG-R1E/MID-MPL (dashed line/circle, n = 5), MIG-R1E9a/MID-MPL (solid line, circle; n = 12), MIG-R1E9a/MID (solid line, open circle; n = 11), MIG-R1E/MID (dashed line, open circle; n = 8), MID-MPL (dotted line, circle; n = 4), and MID (dotted line; n = 4); mice were followed for 210 days (experimental endpoint). (C) Kaplan-Meier survival curve of secondary transplants from MIG-R1E/MID-MPL (dashed line; n = 5), MIG-R1E9a/MID (solid line, circle; n = 3) leukemic cells, and MID-MPL BM (dotted line; n = 4). (D) Kaplan-Meier survival curve of secondary transplantations with MIG-R1E9a/MID (left; n = 6) and MIG-R1E/MID-MPL (right line; n = 6) leukemic cells, treated with vehicle (dashed line) or INCB18242 (solid line), from day 14 after transplantation (arrow).