Treg cells are significantly expanded in LR patients. (A) Gating strategy used to quantify Treg cells. CD4⁺CD25high⁺ T cells (R3) were identified in total lymphocytes (top) and evaluated for their expression of FOXP3 (bottom). (B) Absolute numbers of circulating CD4⁺CD25high⁺FOXP3⁺ cells. Treg total counts were significantly greater in the peripheral blood of LR patients (n = 14) compared both with healthy donors (HD, n = 15) and R patients (n = 10; *P < .001 and P = .018, respectively). Bars represent mean values ± SEM.