Differential expression of the effector molecules perforin and granzymes A, B, and K in CD8⁺ T cells correlates with the intensity of T-bet expression. PBMCs from a CMV-seropositive donor were stained with anti-CD8 and then assessed for the intracellular expression of T-bet, Eomes, granzymes A, B, and K, and perforin. Representative analysis of T-bet and Eomes expression in granzyme A, B, and K and perforin-producing cells is shown as an overlay of total CD8⁺ T cells.