LANA-specific CD4⁺ T-cell recognition of CIITA or control-transduced PELs. The HLA DQ7-restricted GSP-specific clone was incubated in recognition assays with either nontransduced (-), Cont-transduced, or CIITA-transduced (A) JSC-1 or (B) BC-1 PELs. VG-1–nontransduced, control-transduced, or CIITA-transduced PELs were incubated with either HLA DP1-restricted LAP-specific clones (C), HLA DR13-restricted LRS-specific clones (D), HLA DR13-restricted GDD-specific clones (E), or HLA DQ6-restricted LAP/LRS-specific clones (F). In each case, aliquots of target cell lines were also sensitized with 0.1μM of the cognate peptide as controls. Note that this is a lower concentration than that used in Figure 4, which in some cases was insufficient to sensitize targets.