Immunofluorescence analysis of STEVOR protein expression in GIEs. (A) Analysis of STEVOR protein expression in fixed stages II to V GIE preparations. GIEs were acetone-fixed and costained with mouse anti-S2 (green) and rabbit anti-Pfg27 (red) followed by anti–mouse Alexa-488– and anti–rabbit Alexa-594–conjugated IgG. Parasite nuclei were stained with DAPI (blue). Bright-field (BF) and merge images are shown. Bars represent 2 μm. (B) Analysis of live GIEs immunostained with rabbit anti-S2 (green) specifically recognizing native STEVOR on the surface of stages III and IV GIEs. Bound antibody was detected with anti–rabbit Alexa-488–conjugated IgG. BF, nuclear staining (DAPI, blue), and merge images are shown. (C) Immunofluorescence analysis of STEVOR expression in fixed stages II to V-GIEs. GIEs were methanol-fixed and stained with a pool of mouse polyclonal antibodies directed against STEVOR proteins followed by anti–mouse Alexa-488–conjugated IgG (green). Bars represent 2 μm.