General features of MIPs eluted from B-LCLs from 2 HLA-disparate sibships. There were 2 HLA-identical siblings per sibship and each MIP included in the analysis was found in both siblings. (A) Number of MIPs associated with 1, 2, or more different HLA allelic products based on bioinformatic predictions with NetMHC/NetMHCpan. Most MIPs are associated with a single allelic product (HLA type). (B) Predicted MHC I–binding affinity (IC₅₀) of eluted peptides shows that HLA-B–associated peptides are weaker binders than HLA-A–associated peptides (*P < .0001 by 2-tailed Mann-Whitney test). Each dot represents a peptide and the red line corresponds to the mean binding affinity. (C) Venn diagram showing minimal overlap between MIPs from HLA-disparate sibships. Peptide numbers and percentages are depicted for each category. Total number of MIPs for each sibship are shown in colors.