DNase 1 treatment improves blood oxygenation of mice with TRALI. (A) Examples of individual arterial blood oxygen saturation curves recorded in mice with TRALI. The mice received (top panel) intranasal vehicle buffer (n = 11 mice) or (bottom panel) intranasal DNase 1 (n = 11 mice) 10 minutes before anti–H-2Kd antibody injection. Mice that received DNase 1 showed more stable arterial blood oxygen saturation and improved transient hypoxia compared with the mice pretreated with the vehicle buffer. (B-C) Mean (B) and minimum (C) of the arterial oxygen saturation recorded for 20 minutes 2 hours after antibody infusion in control mice (n = 17), mice with TRALI (n = 9), mice with TRALI that have received DNase 1 as a pretreatment 10 minutes before antibody infusion (n = 7; Pre) or as a treatment 90 minutes after antibody infusion (n = 9; Post). Mice with TRALI received intranasally either the vehicle buffer or DNase 1. Bars represent medians. *P < .05. **P < .01. (D) Shock-like condition was determined by rectal temperature measurement in the mice monitored for arterial oxygen saturation and additional control mice (control group: n = 25). Bars represent medians. **P < .01. ***P < .005.
