ATO stabilizes CyPNs and inhibits formation of nuclear PML bodies after progression through mitosis. Live imaging of mCherry-Histone H2B and EYFP-PML1 expressed in HaCaT cells was performed using 2 minutes intervals between image acquisitions for a total imaging period of approximately 20 hours. (A-B) Selected images of control treated cells (A) and cells treated with 0.5μM ATO (B). Circles highlight mitotic cell divisions, and the associated numbers indicate time in minutes before (−) and after (+) metaphase. Scale bar, 20 μm. (C) Illustration of single daughter cells undergoing PML body recirculation after cell division in the absence or in the presence of 0.5μM ATO. The first panels show the cells immediately before mitosis. Images have been synchronized at time 0 in anaphase. Only 1 of the resulting daughter cells is shown. Each of the images represents projections of 3 z-scans. Scale bar, 10 μm. The full movies can be viewed in supplemental Videos 1 and 2.