Tumors from Roquin^san/+ mice exhibit T-cell clonality. T_FH (A) and non-T_FH cells (B) isolated from the lymph nodes of Roquin^san/+ mice with or without tumors and lymph nodes from Roquin^+/+ mice. In mice with tumors, a nonenlarged lymph node was also analyzed for clonality. Cells were purified by flow cytometry. TCRV-β chains were amplified by PCR and clonality determined by CDR3 spectratyping. #Mouse number, as in Table 3. Analysis of non-T_FH cells from Roquin^+/+ mice was not performed. Panels show 5 representative TCRV-β chains selected on the basis of those expressed in most samples. ND indicates not detected. All mice were 26 to 40 weeks of age.