APLN increases expression of hematopoietic markers during hESC differentiation. (A-B) Flow cytometry time-course analysis of CD34, CD43, and GLYCOPHORIN A (GLY) expression on hESCs differentiated in APEL medium supplemented with BVS or BVS with APLN added from day 2. The percentage of cells in each quadrant is shown in the bottom right of each panel. (C) Histogram showing the mean percentage of cells expressing the indicated markers. Error bars represent SEM of 3 independent experiments. Inclusion of APLN resulted in an increased percentage of CD41 (P < .05), CD43 (P < .001), GLYCOPHORIN A (P < .002), and CD33 (P < .05) expressing cells from day 6 of differentiation. Statistical analysis used a 2-way ANOVA with Bonferroni posttest. Detailed FACS plots for all the markers are provided in supplemental Figure 8.