Effect of FG-4497 dose on stabilization of Hif-1α and Hif-2α in the kidney and liver and on serum Epo concentration. Increasing doses of FG-4497 were given orally to wild-type mice on days 1, 3, 5, and 8 and they were killed 6 hours after the last dose. Anti–Hif-1α and Hif-2α Western blots in the kidney and liver (A). ns = nonspecific. The vertical black lines indicate repositioning of lanes from the same gel with the same exposure. Serum Epo concentrations with doses of 6, 20, and 60 mg/kg (B), and 100 mg/kg (C). In panel B n = 2 and in panel C n = 5 for each dose. Error bars represent SEM.