Lnk attenuates phosphorylation of FLT3-WT/ITD. (A) 293T cells were cotransfected with various V5-tagged-Lnk constructs and FLT3. Cells were serum starved for 16 hours (−) and treated with FL for 15 minutes (+). (B) 293T cells were cotransfected with different amount of V5-tagged-Lnk and stable amount of FLT3-ITD and serum starved for 16 hours. Cellular lysates were prepared and precipitated with anti-FLT3 Ab, and both the supernatants and precipitates were subjected to Western blotting analysis using the indicated Abs.