Treg subsets in AA. Treg subsets were studied in 11 AA patients (pretreatment with nonsevere disease) and 6 healthy donors. This figure shows the frequency of CD45RA^+/− and Foxp3^+/− subsets of Tregs. The frequency of Foxp3⁺ CD45RA⁻ (active Tregs; population II and graph in panel B) and Foxp3⁻CD45RA⁺ (resting Tregs; population I and graph in panel A) was decreased in AA (P = .004, P = .01), whereas the frequency of Foxp3⁻CD45RA⁻ subset (cytokine-secreting non-Tregs; population III and graph in panel C) was increased in AA compared with healthy donors (P = .003).