ALPs from Irf8^−/− mice but not wild-type mice produce neutrophils in vivo. (A) LMPPs and ALPs were double-sorted from the BM of Irf8^−/− or wild-type controls and quantitative real-time PCR analysis of IRF-8 expression was carried out. Data are representative of 6 independent experiments. (B-C) Four thousand ALPs purified from either Irf8^−/− or wild-type mice were injected intravenously into each 800 cGy-irradiated F1 recipient (CD45.1⁺CD45.2⁺). BM and spleen were harvested 10 days after transplantation and the development of DCs, neutrophils, and B cells was analyzed by flow cytometry. (B) Representative contour plots. (C) Pooled data from 3 independent experiments. Data represent 5 wild-type and 6 Irf8^−/− recipients. Error bars show calculated SEM; **P < .05.