Effect of plg on IL-6 expression and STAT3 activation in the wound. (A) Comparison of the IL-6 levels between the WT (n ≥ 9) and plg^−/− mice (n ≥ 9) treated with BSA (control) or plg. (B) Western blot analysis of the pSTAT3 levels in skin lysates from the unwounded WT mice, wounded WT mice, and wounded WT mice treated with plg (n ≥ 5). β-actin served as a loading control. The level of pSTAT3 was quantified using ImageJ Version 1.41o software. Values are expressed as the intensity of pSTAT3 divided by the intensity of total STAT3. ns indicates not significant. *P < .05; **P < .01.