Confocal analysis of erythroblasts, enucleating erythroblasts, and reticulocytes with Abs to organelle and plasma membrane marker proteins. All cells were cultured following protocol A. Cells were fixed in 1% (wt/vol) paraformaldehyde and permeabilized with 0.05% (wt/vol) saponin. (A) Cells were harvested on day 12 and stained for the presence of the plasma membrane markers glycophorin A, glycophorin C, Rh polypeptides, β1 integrin, CD98, and CD147. (B) Cells were stained for the presence of the organelle and cytosolic markers LAMP1 (lysosomes), calreticulin (ER), CD63 (endosomes), giantin (Golgi), pericentrin (centrioles), and tubulin. (C) Cells were stained for the presence of filamentous F-actin. Scale bars indicate 5 μm.