Enrichment of CMPs in Evi1^pos clonal MLL-AF9–transformed BM cell cultures. (A) Example of FACS-sorting strategy to select for CMP, GMP, and MEP derived from cultured Evi1^pos and Evi1^negMLL-AF9 clones. (B) Percentages of CMP, GMP, and MEP relative to the total number of progenitor cells are calculated for each clone. (C) Evi1 expression in LSK, CMP, GMP, and MEP subpopulations of normal mononucleated mBM cells. The MLL-AF9 cell line 4166 served as positive control for Evi1 expression. (D) Evi1 mRNA expression in lineage-positive subfractions of MLL-AF9 clones. The expression of Evi1 was calculated relative to the lineage-negative fraction of MLL-AF9 clone #1. Evi1 relative expression was normalized using Hprt as a reference gene. For the last 2 panels, the average of 3 experiments with its SD is shown.