Effect of NIF expression on VWF levels. Blood was collected from mice 5 days before and 4 days after hydrodynamic gene transfer with pLIVE-NIF in macLRP1⁺ mice (n = 10) or macLRP1⁻ mice (n = 5). Plasma was prepared and analyzed for FVIII activity (A) and VWF antigen (B). Data are presented as percentage of pretreatment levels. FVIII and VWF levels before and after treatment were assessed for statistical significance using a paired t test. A statistically significant increase in FVIII and VWF levels was found in macLRP⁺ mice but not in macLRP1⁻ mice.