CD62L− T cells facilitate hematopoietic cell engraftment after allogeneic TCD BM transplantation. TCD BM cells from B6 CD45.2 (5 × 105) and CD62L− T cells (1 × 106) from unprimed B6 CD45.1 mice were transplanted into lethally irradiated BALB/c recipients. Mortality was recorded daily. Each group contained 5 to 10 animals (TCD BM only, 10; CD62L−, 10; and contaminating, 5). The data are the representative of 2 independent experiments with similar data. (A) Survival. CD62L− versus TCD BM only or contaminating (P < .01); TCD BM only versus contaminating (P = .86). (B) Hematologic recovery. The recovery of white blood cells (WBCs) and platelets was analyzed in peripheral blood at day 30 after transplantation. All values represent means + SD (*P < .05; CD62L− vs TCD BM only or contaminating in both WBC and platelets). The mean values of WBCs and platelets in peripheral blood of normal BALB/c mouse (age, 8-12 weeks; female) were 6.1 ± 1.5 K/μL and 716.1 ± 142.4 K/μL, respectively. (C) Chimerism analysis. Chimerism in different cell subsets was analyzed in peripheral blood by flow cytometry on day 30 after transplantation. All values represent means + SD (*P < .05; CD62L− versus TCD BM only or contaminating in all cell subsets except CD62L− vs contaminating in CD19+ cells).
