Figure 4
Figure 4. Surface phenotype of CD56brightCD16−/low, CD56dimCD16+, and CD56−CD16+ NK-cell subsets reconstituting after intrabone UCBT. Purified NK cells were stained with anti-CD56 and anti-CD16 mAbs to identify 3 subsets: CD56brightCD16−/low, CD56dimCD16+, and CD56−CD16+. Each of the 3 gated populations was assessed for the expression of the indicated surface Ags. In each histogram the percentage of positive cells is shown. The number in italics indicates the percentage of CD56dim or CD56−CD16+ NK cells expressing the CD94dim phenotype. A representative patient of group 1 and 1 of group 3 are shown in the first and second panels, whereas a CB and a healthy adult are shown in the third and fourth panels, respectively. The phenotype of CD56−CD16+ is not reported for group 3 patients and adult donors because in these 2 cases this NK subset was substantially undetectable.

Surface phenotype of CD56brightCD16−/low, CD56dimCD16+, and CD56CD16+ NK-cell subsets reconstituting after intrabone UCBT. Purified NK cells were stained with anti-CD56 and anti-CD16 mAbs to identify 3 subsets: CD56brightCD16−/low, CD56dimCD16+, and CD56CD16+. Each of the 3 gated populations was assessed for the expression of the indicated surface Ags. In each histogram the percentage of positive cells is shown. The number in italics indicates the percentage of CD56dim or CD56CD16+ NK cells expressing the CD94dim phenotype. A representative patient of group 1 and 1 of group 3 are shown in the first and second panels, whereas a CB and a healthy adult are shown in the third and fourth panels, respectively. The phenotype of CD56CD16+ is not reported for group 3 patients and adult donors because in these 2 cases this NK subset was substantially undetectable.

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