THP-1 HBG1/HBG2 expression after PLP RNase treatment. Cells cocultured for 24 hours with RNase-treated PLPs expressed less HBG1/HBG2 (RNaseA/T1 and RNase ONE) compared with the untreated PLP coculture (PLP). HBG1/HBG2, normalized against GAPDH, was absent in the control, THP-1 cells treated with 1 μg/mL PAM and 100 ng/mL TPO.