Adhesion and shape change. (A) Shape change of representative primary CD4 T cells before (−) or 60 seconds after (+) SDF-1 stimulation. Midplane confocal images stained for actin (phalloidin, red) and for the transgenic protein (using anti-HA, green). (B) Scoring of populations of cells from panel A (n = 30) for their shape 5 minutes after SDF-1 stimulation. Because the elliptical form factor scores = length of longest axis/length of axis perpendicular to that, a value of 1.0 is a round cell, and higher values are progressively more elliptical (P < .001). (C) Analysis of asymmetry of transgenic protein. Lymphocytes were stimulated with SDF-1 and stained with anti-HA to detect localization of WT or T567E transgenic protein. For each cell, average pixel intensity was compared between the front and the back. Data are the mean ± SE of values for 70 cells each (P < .0.05). (D-E) Adhesion of CD4 T-lymphoblasts to immobilized VCAM-1 or ICAM-1 in the absence (D) or presence (E) of Mn²⁺.