Ezrin T567E transgenic lymphocytes have increased membrane tension. (A) Representative single tether force traces are shown for WT and ezrin transgenic T567E T-cell blasts. (B-C) Bar graphs showing mean ± SE of the PTF and the SSTF from WT (solid fill) and T567E (white fill) T-cell blasts. (WT PTF, 75.9 ± 15.7; WT SSTF, 36.8 ± 7.1; T567E PTF, 107.8 ± 18.5; T567E SSTF, 64.8 ± 10.9). *Populations are significantly different (P < .05). The PTF represents the force required for successful formation of a membrane tether. The PTF has a small contribution from the force required to overcome the membrane's resistance to a change in shape and a larger contribution from the force required to break membrane-cytoskeleton adhesion bonds.³²