Retrovirally transduced normal HSCs sustain myelopoiesis after Stat5 deletion. Genomic DNA from BM of recipients of MIGFP-transduced Stat5a/b^fl/− and Mx-Cre;Stat5a/b^fl/− donor BM was isolated 2 months after transplantation and analyzed by Southern blot for efficiency of Stat5a/b deletion and number of engrafting proviral clones, as in supplemental Figure 1C. Top panel: Analysis of efficiency of recombination of the floxed Stat5a/b allele in recipients of MIGFP-transduced Stat5a/b^fl/− BM (lanes 3-7) and in untreated (lanes 8-9) and pIpC-treated (lanes 10-12) recipients of MIGFP-transduced Mx-Cre;Stat5a/b^fl/− BM using a Stat5 probe. Bottom panel: Analysis of the number of engrafted retrovirally transduced HSC clones in the 3 cohorts using a GFP probe.