VH and Vκ gene positions relative to CTCF occupancy. (A) Overview of VH gene positions relative to the nearest CTCF binding site for the mouse Igh locus. The germline Igh locus is shown with the locations of VH, DH, JH, CH, and enhancer elements (top). VH genes were grouped according to the location of the nearest CTCF site and whether this site was located upstream (−) or downstream (+) relative to VH transcriptional orientation. Color-coded groups of VH genes were plotted at their respective locations below the germline VH genes. On the right, a pie-chart represents the distribution of the different VH gene groups expressed in percentages of the total number of VH genes. (B) Identical to panel A, but for the Vκ genes within the mouse Igκ locus.
