CCL-3 expression is increased in malignant cells from leukemic mice. (A) Top panel: Representative wells from CFU-OB cultures stained for alkaline phosphatase activity (pink). Bottom panel: CCL3 levels in culture media from CFU-OB cultures. (B) CCL3 protein levels in murine model of AML compared with normal controls. (C) Relative expression of CCL3 in bone marrow mononuclear cells isolated from whole bone marrow, cells sorted for GFP and YFP expression according to Figure 1D, and cells liberated from bone fragments by collagenase digestion and magnetically separated based on CD45 cell surface expression. (D) Relative expression of human CCL3 in primitive CD34⁺CD38⁻CD123⁺ AML cells compared with normal controls. Each bar represents a single AML sample normalized to 3 normal controls. (E) CCL3 protein levels in human AML patient marrow plasma. Each bar represents a single AML marrow sample compared with 7 normal controls. *P ≤ .05. ***P ≤ .001. (A-B,D) Each data point represents an individual mouse. (C) n = 3 mice per treatment group.