Comparison of PDI expression and platelet accumulation during thrombus formation. Rabbit polyclonal anti-PDI antibody conjugated to Alexa Fluor 488 (0.3 μg/g body weight) and Fab fragments of anti–CD-41 antibody conjugated to Alexa Fluor 647 (0.2 μg/g body weight) were infused into the mouse 5 minutes before arteriolar injury. In certain conditions, eptifibatide (10 μg/g body weight) was infused before injury and reinfused every 20 minutes for subsequent thrombi. (A). Representative binarized images of the appearance of fluorescence signals associated with PDI (green) and platelets (red) over 180 seconds after laser-induced vessel-wall injury in a wild-type (WT) mouse (left panels) or a wild-type mouse treated with eptifibatide. (B) Median integrated platelet fluorescence. Median fluorescence is presented vs. time after vessel-wall injury. Curve 1, WT mice; curve 2, WT mice treated with eptifibatide. (C-D) Median integrated PDI fluorescence detected by rabbit affinity-purified anti-PDI antibody. (D) Wild-type mouse treated with sodium beraprost (30 μg/kg body weight). Curve 1, WT mice; curve 2, WT mice treated with sodium beraprost.