ARHGAP25 expression in human tissues and in leukocytes. (A) Domain structure of ARHGAP25. (B) Northern blot analysis of the tissue distribution of ARHGAP25 mRNA. Human multiple-tissue Northern blot was hybridized with radiolabeled full-length ARHGAP25 cDNA as a probe. (C) Analysis of the expression of ARHGAP25 protein in leukocytes isolated from human peripheral blood (PMN, PBMC) or from human tonsils (T cells and B cells), using purified polyclonal ARHGAP25 antibody. WCL means whole cell lysate. Negative and positive controls are provided in Figures 3A and 4B and supplemental Figure 1.