Similar 3-week platelet and neutrophil outputs from Lin⁻ALDH⁺ progenitors in human CB and mPB detected by a clinically relevant xenotransplantation assay using NSG mice. (A) The individual values shown are average outputs calculated for different samples of CB and mPB based on limiting dilution analysis of the frequency of rapid platelet- and neutrophil-producing cells present in each sample tested and their measured average outputs at doses that were nonlimiting but also nonsaturating. The crossbars represent the geometric mean ± SEM of the sample values for CB and mPB separately. (B) Poor rapid human platelet production in NSG mice correlates with delayed platelet recovery in clinical transplantations. Shown is a plot of the number of rapid human platelet-producing cells (measured using the 3-week NSG transplantation assay) determined to be present in 42 mPB autotransplantations (based on the frequency of these multiplied by the cellularity of the transplantation and then normalized per kilogram of body weight of the patient) versus the time taken by each patient to recover their platelet count (defined as > 20 × 10⁹/L after transplantation). The dotted line shows the definition of delayed recovery chosen here to be 12 days to correspond to the earliest time when human platelets would appear from the cells assayed in NSG mice.