CD161⁺⁺CD8α⁺ T cells in cord blood have broad T-cell receptor usage compared with adults. (A) Comparison of proportional TCR Vβ usage by CD161⁺⁺CD8α⁺ T cells between cord and adult blood using TCR Vβ antibody panel. Data obtained from 8 cord blood samples and 10 adult healthy donors. (B) Representative FACS plot of Vα7.2 usage by CD161^++/+/− subsets in cord blood (C) Comparison of TCR Vα 7.2 usage between the CD161⁺⁺CD8α⁺ subsets in cord and adult blood. ***P = .002 (Mann-Whitney).