Distinct phenotypic and functional features of the CD161⁺⁺CD8α⁺ population are shared between the CD161⁺⁺ CD8αα⁺ and CD8αβ subsets. (A) Representative histograms showing CD161⁺⁺ CD8αα⁺ and CD8αβ cells to express CCR6, CCR2, and CXCR6. Cumulative data shown. ns = non significant (Mann-Whitney test). (B) Cumulative data showing expression of IFN-γ, IL-17 and IL-22 by CD161⁺⁺ CD8αα and CD8αβ cells. *P = .0467, ns = non significant (Mann- Whitney). (C) Heat-map showing up (red) and down (green) regulated gene expression of CD161⁺⁺CD8αα (light blue) compared with CD8αβ (dark blue) cells on microarray.