MAIT cell and CD161⁺⁺CD8⁺ populations are overlapping subsets. (A) CD3⁺CD161⁺⁺Vα7.2⁺ T cells are predominantly CD8αα⁺ and CD8αβ⁺ cells. Left hand panel shows representative FACS plots of coreceptor usage by Vα7.2⁺ T cells. Cumulative data shown in right hand panel. ***P < .001 (1-way ANOVA). (B) Representative FACS plots show a prominent CD8α⁺CD8β⁻ population exclusive to the CD161⁺⁺CD8α⁺ subset in peripheral blood. Data representative of 20 healthy donors. (C) CD161⁺⁺CD8α⁺ T cells are predominantly Vα7.2⁺ (mean 87% Vα7.2⁺) compared with the CD161⁺ and CD161⁻ CD8α⁺ subsets. **P < .001 (1-way ANOVA).