T-cell activation measured by the proliferation antigen Ki67 that changed significantly relative to baseline (day −21 and day 0) only in CD8⁺ T cells sampled from peripheral blood. The levels of Ki67 on CD8⁺ (A-C) and CD4⁺ (D-F) T-cell subsets in PBMCs (A,D), RBs (B,E), and LNs (C,F) were determined by flow cytometry and analyzed using the Friedman test with Dunn multiple comparisons.