Hematopoietic markers analysis of HC, EC, and DP fractions in the human EL. (A) Whole liver cells from a 7.7-week-old human EL were double-stained with anti-CD45 and -VE-cadherin mAbs. Flow cytometric analysis demonstrating endothelial (VECAD^highCD45⁻ [b] and VECAD^lowCD45⁻ [c]), DP (VECAD^lowCD45^low [d]), and hematopoietic (VECAD⁻CD45^high [e] and VECAD-CD45^low [f]) cell populations. Numbers indicate the percentages of positive cells in the corresponding gates. Data are representative of 3 independent experiments. (B) Whole liver cells from 7.0- to 8.4-week-old human ELs triple-stained with anti-CD45, anti-VE-cadherin, and anti-hematopoietic-specific mAbs. Relative expression of HC surface antigens by total [a], endothelial (VE-cadherin^highCD45⁻ [b] and VE-cadherin^lowCD45⁻ [c]), DP (VE-cadherin^lowCD45^low [d]), and hematopoietic (VE-cadherin⁻CD45^low [e] and VE-cadherin⁻CD45^high [f]) cell populations. Numbers indicate percentages of positive cells in the corresponding quadrants. Data are representative of at least 3 independent experiments.