Activation of the FOXO target genes. KM-H2 and L428 cell lines expressing FOXO1(A3)ER were treated or not treated with 200nM 4-OHT. Twenty-four hours later, cells were harvested and TNFSF10, PMAIP1, BCL2L11, and CDKN1B mRNA expression was assessed as described in Figure 2. ND indicates not detected; and EV, empty vector. Data are mean of relative expression ± SD. The measurements were done in triplicates.