Removal of p-preDCs from FCs abrogates the induction of chimeric Treg generation. (A) Sorted 45 000 FCs, from which the p-preDC subpopulation had been removed, were transplanted with 10 000 B6 HSCs + 1000 NOD HSCs into conditioned NOD recipients. (B-D) Representative analysis of recipient CD8⁻/CD4⁺/CD25⁺/FoxP3⁺ Tregs in spleen, thymus, and bone marrow at 5 weeks after transplantation. (E) The absolute number of recipient-derived Tregs in peripheral blood, spleen, thymus, and bone marrow from mice that received HSC + FC (n = 4) and HSC + FC without p-preDCs (n = 4) 5 weeks after transplantation. Results are means ± SEM.