CD8⁺/TCR⁻ FCs enhance HSC engraftment in NOD mice (B6 → NOD). The ability of B6 FCs to promote B6 HSC engraftment in allogeneic NOD recipients and long-term survival were evaluated. (A) Percent engraftment of NOD mice that received 10 000 B6 HSCs + 30 000 B6 FCs or 45 000 B6 FCs. (B) Percentage of donor chimerism in NOD recipient mice with engraftment. Dot plots represent percentage of donor chimerism in individual animal. Lines indicate median percentages. *P = .22; **P = .006. (C) Survival of NOD recipients conditioned with 950 cGy of TBI and transplanted with 10 000 B6 HSCs + 30 000 FCs (□), 10 000 B6 HSCs + 45 000 B6 FCs (▲), or 10 000 B6 HSCs alone (●). Results are shown for survival data from 3-5 separate transplant experiments.