Inhibition of c-MYC expression blocks the induction of c-MYC–dependent M2 markers. (A-B) Effect of cycloheximide (open bars) and vehicle (closed bars) on the induction of genes associated with alternative activation in human macrophages exposed to 20 ng/mL of IL-4 for 24 hours. Results are shown as means ± SD of n = 3 independent experiments. (C-D) Effect of c-MYC/shRNA (open bars) and mock/shRNA (closed bars) on the induction of genes associated with alternative activation in human macrophages exposed to 20 ng/mL of IL-4 for 24 hours. Results are shown as means ± SD of n = 3 independent experiments. (E) Expression levels of 12/15-Alox, Mcr1, Maoa, Wnt5a, Apak12, and Pcsk5 transcripts evaluated by Q-PCR in peritoneal murine macrophages isolated from c-Myc-fl/c-Myc-ERKI mice exposed to adeno-cre for 72 hours after stimulation for 24 hours with medium, 20 ng/mL of IL-4, 100nM 4-HT, or 20 ng/mL of IL-4 plus 100nM 4-HT. Results are shown as means ± SD of n = 3 independent experiments with 3 animals used in each experiment.