c-MYC–dependent genes in M2 macrophages. Microarray analysis was conducted on 3 independent preparations of resting macrophages (M0) and macrophages exposed to 20 ng/mL of IL-4 for 24 hours in the presence or absence of the c-MYC inhibitor 10058-F4 (60μM; M2 inh and M2, respectively). Hierarchical clustering of genes induced by IL-4 (fold of induction ≥ 2 and P ≤ .05 on a 2-tailed paired Student t test comparing M2 vs M0) with induction significantly affected by c-MYC inhibition is shown (P ≤ .05 on a 2-tailed paired Student t test comparing M2 inh and M2). Genes not significantly affected by c-MYC inhibition are shown in supplemental Figure 3. Columns report gene names in the HUGO nomenclature, the -fold of increase of genes in M2 versus M0 in the absence or presence of c-MYC inhibitor (M2/M0 ratio and M2 inh/M0 ratio, respectively), and the percentage and statistical significance of inhibition of gene induction in the presence of c-MYC inhibition.