Myelosuppression induces TIMP-3 in the bone marrow. (A) Induction of TIMP-3 by BM suppression. Mice (C57BL/6) were intraperitoneally injected with 150 mg/kg of 5-FU, and BM mononuclear cells (BMMNCs) were harvested at the indicated time points. RNA was extracted and examined for the expressions of TIMP-3 by Q-RT-PCR. Data were normalized and analyzed by comparative C_T method and shown as relative values to day 0. Data are mean ± standard deviation (SD) (n = 3). (B) Induction of TIMP-3 by irradiation. Mice were treated with lethal irradiation (950 R), and BMMNCs were harvested at the indicated time points. The expression of TIMP-3 was examined by Q-RT-PCR. Data were normalized and analyzed by comparative C_T method and shown as relative values to day 0. Data are mean ± SD (n = 3).