Both IL-29 and IFNα up-regulate TLR8 mRNA expression. Monocyte-derived macrophages were stimulated with medium, IL-29, or IFNα for 5 hours, and then the mRNA expression of TLR7 and TLR8 was measured by RT-PCR. B cells, monocytes, and monocyte-derived DCs were included as controls. The values depicted show representative data from 5 independent experiments.