Iron absorption genes are decreased in Hepc^−/−Hif-2α^Δint compared with Hepc^−/− mice. (A) Relative mRNA expression of DMT1 + IRE, DCYTB, and FPN normalized to Cyclophilin in the duodenum of Hepc^−/−Hif-2α^Δint (●; n = 9) versus Hepc^−/− (■; n = 9) and CTR (▴; n = 9) mice. (B) Western blot of FPN, DMT1, and DCYTB on membrane extracts of whole duodenum from Hepc^−/−Hif-2^Δint and Hepc^−/− mice versus CTR littermates. Expression was normalized to β-actin. Results were quantified using ImageJ Version 1.43r software (http://rsb.info.nih.gov/ij/). All genotypes used contain the Hif-2α^lox/lox allele. *P < .05. **P < .01. ***P < .001. ns indicates not significant.