Genes showing association of transcript abundance in platelets with platelet response. (A) Sixty-three transcripts in platelets that show a correlation between microarray signal intensity (SI) and functional responses. Genes are grouped alphabetically and by cellular location of the encoded proteins. Platelet activation was measured as fibrinogen binding (F) and P-selectin expression (P) in response to ADP (A) or the collagen mimetic, CRP-XL (C). Correlation with functional responses for each transcript is indicated by the colored squares, with positive and negative correlations indicated by the diagonal lines. The 6 genes selected for genotyping in the cases of myocardial infarction (MI) and controls are highlighted in light blue and bold upper case. (B) Correlation between platelet transcript level and platelet function, in 37 subjects selected for the whole-genome expression study, for the 2 genes showing association with MI. SI was measured with Illumina probes GI_29789284-S and GI_4758689-S for COMMD7 and LRRFIP1, respectively. Platelet activation is shown as the standardized residuals of logit transformed flow-cytometric measurement of the percentage of platelets positive for specific markers of activation, as previously described.1 Graphs display the correlation with the highest significance, and boxes show Pearson correlation coefficients (r) and probability values (in parentheses) for all 4 functional parameters, with the highest significance shown in bold.
