Modulation of LB-ADIR-1F–specific recognition by IFN-α. Recognition of MNCs from 3 different LB-ADIR-1F–positive donors was measured by direct cytotoxicity in 4-hour CFSE assays (A) and by 24-hour IFN-γ release (B) following preincubation in medium alone or in medium containing 1000 IU/mL IFN-α for 48 hours (shown as mean value ± SD). Recognition of MNCs (▪) and MNCs exogeneously pulsed with saturating concentrations of synthetic peptide (□) was measured at an E/T ratio of 1:1. Only minimal up-regulation of recognition in IFN-α precultured MNCs was observed.