Enhanced transwell migration in PTEN^−/− neutrophils. WT and PTEN^−/− neutrophils (0.5 × 10⁴) were allowed to migrate in response to the indicated concentration of (A) fMLP, (B) IL-8, and (C) C5a in transwell systems. Percentage of cells that migrated into the bottom well was recorded. Data shown are mean ± SD of 3 wells, from 1 experiment representative of 3. *P < .05 versus wild-type neutrophils.