American Society of Hematology

Figure 1

$Figure 1. KGF increases thymocyte numbers via induction of TN cell division. Adult naive C57BL/6 mice were treated with KGF (5 mg/kg, ⊡) or HBSS (▪) on 3 consecutive days (days 0, 1, and 2), and thymi were analyzed by flow cytometry at the indicated days. (A) Total thymocyte numbers (cells × 10−6). (B-F) TN cell numbers per thymus (× 10−6). TN indicates CD3−4−8−; TN1, CD44+CD25−; TN2, CD44+CD25+; TN3, CD44−CD25+; and TN4, CD44−CD25− cells. (G-J) BrdU incorporation into TN cells as a measure of cell division. The data represent the fraction (in %) of cells among total thymocytes that have incorporated BrdU. (K-M) Total numbers per thymus of CD4+CD8+, CD4+CD8−, and CD4−CD8+ cells. Three independent experiments were performed, and one representative experiment is shown. Mean ± SD; *P < .05 versus HBSS controls, with 5 mice per group and time point.$

KGF increases thymocyte numbers via induction of TN cell division. Adult naive C57BL/6 mice were treated with KGF (5 mg/kg, ⊡) or HBSS (▪) on 3 consecutive days (days 0, 1, and 2), and thymi were analyzed by flow cytometry at the indicated days. (A) Total thymocyte numbers (cells × 10⁻⁶). (B-F) TN cell numbers per thymus (× 10⁻⁶). TN indicates CD3⁻4⁻8⁻; TN1, CD44⁺CD25⁻; TN2, CD44⁺CD25⁺; TN3, CD44⁻CD25⁺; and TN4, CD44⁻CD25⁻ cells. (G-J) BrdU incorporation into TN cells as a measure of cell division. The data represent the fraction (in %) of cells among total thymocytes that have incorporated BrdU. (K-M) Total numbers per thymus of CD4⁺CD8⁺, CD4⁺CD8⁻, and CD4⁻CD8⁺ cells. Three independent experiments were performed, and one representative experiment is shown. Mean ± SD; *P < .05 versus HBSS controls, with 5 mice per group and time point.

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