Thrombin neither activates platelet sGC nor induces VASP phosphorylation. Platelets from wild type (WT) and platelet-specific sGC KO mice (PSGC-KO) were isolated exactly according to the protocol used by Zhang et al¹  and incubated with the indicated concentrations of thrombin (5 minutes) or 10μM of SNP (1 minute). Parts of the samples were used for cGMP measurement and the rest for Western blot analysis of VASP, p38, and ERK phosphorylation. Results are representative of 5 independent experiments.